Le trait d'union of my scientific research is the application of state-of-the-art optical and physical techniques to unravel cellular regulation mechanisms at the single-molecule level. I am particularly interested in transcription regulation and in the interplay between nuclear factors mobility, genome organization, and DNA topology in orchestrating gene expression plasticity. Currently, I work on a project dedicated to study the target search mechanisms of individual DNA-binding proteins in human cells. In my future researches, I want to encompass the study of endogenous biochemical reactions and to investigate how complex molecular machinery are recruited and assembled at specific sites on the genome, in living cells. Also, I am very interested in understanding how the “linear” and the “three dimensional” organization of genes in functional territories as well as in different chemical and physical environments influences the nuclear processes.